Please use this identifier to cite or link to this item: http://hdl.handle.net/11717/6044
Title: 1-Naphthol as an excited state proton transfer fluorescent probe for erythrocyte membranes
Authors: Pappayee, N.
Mishra, A.K.
Keywords: Biological membranes
Electron energy levels
Emission spectroscopy
Fluorescence
Lipids
Phase transitions
Protons
Erythrocyte
Naphthol
Phospholipids
Proton transfer
Cholesterol
1 naphthol
1-naphthol
cholesterol
fluorescent dye
liposome
naphthol derivative
chemistry
erythrocyte membrane
human
metabolism
spectrofluorometry
temperature
Cholesterol
Erythrocyte Membrane
Fluorescent Dyes
Humans
Liposomes
Naphthols
Spectrometry, Fluorescence
Temperature
Issue Date: 2000
Publisher: Elsevier Science B.V., Amsterdam, Netherlands
Citation: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 56(11), 2249-2253
Abstract: The microenvironmental dependence of excited state prototropism of 1-naphthol and the corresponding changes in its fluorescence emission is utilized to monitor the acyl chain melting phase transition behavior of liposome membrane made from human erythrocyte lipids. A sharp increase in the ratio of neutral/anionic form fluorescence intensity is noticed at the phase transition temperature (19 �C). This provides a convenient method for obtaining phase transition temperature in lipid membranes. The membrane modifying effect of cholesterol on the erythrocyte liposome is successfully sensed by 1-naphthol fluorescence.
URI: http://dx.doi.org/10.1016/S1386-1425(00)00312-7
http://hdl.handle.net/11717/6044
ISSN: 13861425
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