Rapid relase of protoplasts from Eremothecium ashbyii in comparison with Trichoderma reesei and Penicillium chrysogenum using Novozyme and Funcelase

Protoplast release in Eremothecium ashbyii, Trichoderma reesei, and Penicillium chrysogenum was achieved using commercially available enzymes, Novozyme 234 and Funcelase. A rapid release of protoplasts was observed in E. ashbyii, yielding nearly 4.0 × 107 protoplasts ml-1 in 10-35 min. The regeneration frequency of protoplasts from T. reesei, P. chrysogenum, and E. ashbyii using Funcelase was 51.77, 28.32, and 7.64%, respectively, and was higher in comparison with Novozyme-derived protoplasts. © 1993.