Chemometric optimisation of parameters for biocatalytic reduction of copper ion by a crude enzyme lysate of Saccharomyces cerevisiae grown under catabolic repression conditions

The capacity of the microbes to reduce the metal has been demonstrated. The immobilised induced microbes with toxic chemical CuCl2 was used to reduce the Cu ions as elemental metal and by using the response surface methodology the parameters such as the inducer concentration, the time of inducer addition which are concerned with the growth and formation of specific enzymes and the initial substrate concentration, initial pH of the substrate solution and the time of reaction which are concerned with the biocatalytic reduction of the metal ions were optimised for maximum reduction. The elemental copper reduced and removed experimentally from its ionic state at the optimum conditions was 54.82 ppm. © 2001 Elsevier Science B.V.