Microbial Synthesis of Hydroxyapatite-Nanocellulose Nanocomposites from Symbiotic Culture of Bacteria and Yeast Pellicle of Fermented Kombucha Tea

The strong need for the utilization of industrial by-products and biowaste increases as we transition towards a circular economy. On these grounds, the present research aims to explore the applicability of the Symbiotic Culture of Bacteria and Yeast (SCOBY), a by-product of a functional beverage industry, for applications in biomedicine. Herein, hydroxyapatite (HA)-coated SCOBY nanocellulose (SN) nanocomposite (SNHA) was synthesized via a novel biomimetic approach using Serratia marcescens strain by adopting two different in situ approaches. Characterization studies estab-lished the presence of functional groups corresponding to pure nanocellulose and HA. Microscopic analysis revealed SN fibers of the dimensions 30–50 nm surrounded by 10–15 nm rod-shaped HA crystals. The SNHA membranes were carbonated and harbored traces of metal ions. A deposition of nano-HA crystals as high as 30–50% was achieved. Overall, the synthesized SNHA membranes reflected increased stability, low crystalline nature and an ion-substituted structure resembling the natural bone; they are thereby suited for bone tissue engineering.