Effects of probe placement on tissue oxygenation levels during reflectance measurements for different types of tissues in a clinical setting

Diffuse reflectance spectroscopy (DRS) have been investigated as an alternate technique to tissue histopathology for the early detection of premalignant lesions in a wide variety of tissues. Spectroscopic techniques require a fiber optic probe to be kept in contact with a biological tissue at an optimal distance to illuminate and receive light during spectral measurements. This probe-contact pressure introduces artifacts in optical properties depending on the biological and morphological status of the tissue. The objective of this investigation is to study the effects of probe pressure on different types of tissues with varying anatomical structures on tissue oxygenation levels during reflectance measurements.