A FED-BATCH SURFACE CULTURE PROCESS FOR THE PRODUCTION OF CITRIC-ACID WITH REUSE OF ASPERGILLUS-NIGER MYCELIA

Citric acid production in surface culture process with reuse of mycelia for more than one batch of media has been reported in this work. A strategy for replacing media and thus increasing productivity in subsequent batches is discussed. An explanation for the decrease in critic acid accumulation beyond the second batch is suggested on the basis of oxygen limitation.