Determination of quenching inhibition factor and selective fluorescence quenching study of perylene, pyrene and fluoranthene in micelle by cetyl pyridinium chloride as a hydrophobic quencher molecule

The fluorescence quenching study of perylene, pyrene and fluoranthene by cetyl pyridinium chloride (CPC) a hydrophobic quencher molecule has been carried out using synchronous fluorescence scan. A quenching inhibition factor (QIF) has been defined for added quencher in inert micellar medium. The other quenching parameters calculated are KQ and EQ. The obtained quenching parameter QIF well compares with these values. The quenching mechanism is found to be explainable by a quencher sphere of action model. In micellar medium the fluorescence quenching efficiency is in the order: pyrene > perylene > fluoranthene. A correlation between alternate and non-alternate PAHs and aromatic ring size with QIF has been reported for CPC in micelle.