Whole Cells Mediated Biocatalytic Reduction of Alpha-Keto Esters: Preparation of Optically Enriched Alkyl 2-hydroxypropanoates

Biocatalytic reduction of alkyl 2-oxopropanoates were carried out by utilizing the whole cells of Candida parapsilosis ATCC 7330 to form the optically enriched alkyl 2-hydroxypropanoates with good enantiomeric excess (ee) (≤91%) and isolated yields (≤68%). Enantiomerically enriched (S)-ethyl 3-bromo-2-hydroxypropanoate thus synthesized by biocatalytic reduction of ethyl 3-bromo-2-oxopropanoate is presented in this study for the first time in water under ambient reaction conditions in a reaction time of 4 h which is considerably less than earlier reported procedures.