Constriction based microfluidic device for the cell phenotyping

We report a constriction based microfluidic device for characterization of mechanical properties of single cells. The device is capable of measuring the effect of size ratio, entry angle as well as external pressure applied across the constrictions on cell movement. Multiple cells can be processed through the constrictions at a single stretch. The device can give much higher throughput than the single constriction device normally used for all the experiments. It was observed, the entry time increase with the decrease in the cone angle (entry angle). Experimentally, the velocity of the cell was found to increase along the constriction length.