Electroporation: Basic principles, practical considerations and applications in molecular biology

There are many approaches available for introducing foreign DNA into eukaryotes and prokaryotes. The most commonly used technique and currently of more practical interest is the electroporation. This review will focus on the electroporation as a promising, highly efficient and effective means of gene transfer. We summarize a detailed assessment of the various parameters and conditions that govern electroporation of a wide range of cell types.