Light-Induced Cellular Delivery and Analysis

In the era of advanced nanotechnology, laser-assisted photoporation is of particular importance for the delivery of membrane impermeable biomolecules into living cells for advanced biological and biomedical research. It has the advantage of briefness and nontoxicity. Photoporation is based on the localized transientpore generation in the cell membrane using pulsed or continuous laser light. Membrane permeability can be increased directly by focused laser light, with the help of micro-fabricated devices or in combination of sensitizing nano particles for higher throughput delivery. Here in this chapter, we have presented anoverview toward single-cell photoporation/optoporation mechanisms and their biological and therapeutic applications.