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Tuhin Subhra Santra
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Tuhin Subhra Santra
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Tuhin Subhra Santra
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Santra, Tuhin Subhra
Santra, T. S.
Santra, Tuhin
SANTRA, Tuhin Subhra
Santra, Tuhin S.
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71 results
Now showing 1 - 10 of 71
- PublicationNano-focused electric field for nano-localized single cell electroporation using ITO nanoelectrode chip(01-01-2019)
; ;Kar, S.Tseng, Fan GangBiomolecular delivery into single living cell with high transfection efficiency and high cell viability is of great interest for cellular research, therapeutics and diagnostics purpose. Here we demonstrate localize single cell electroporation using Indium Tin Oxide (ITO) based nanoelectrode chip, which is a compact, easy to use, with low voltage applications. We fabricated 40 array of nanoelectrodes on top of the silicon surface by using lithographic process and the gap between two nanoelectrodes were 70 nm. Due to apply sufficient external voltage, cell membrane deform and biomolecules gently deliver into cells. The best result was achieved using Propidium iodide dye with 94% delivery efficiency and 98% cell viability for AGS cell line. - PublicationThe physics of terahertz negative photoconductivity in low-dimensional materials(01-03-2022)
;Kar, S. ;Lake, J. ;Adeyemo, S. O.; Joyce, H. J.Enhancement of conductivity is the common photoresponse when incident photons temporarily generate free carries after photoexcitation in a solid-crystalline-material. In sharp contrast, some emerging low dimensional materials such as graphene, transition-metal dichalcogenides, topological insulators, MXenes, and carbon nanotubes possess reduced terahertz-range conductivity after photoexcitation, a phenomenon that has attracted significant interest in the research community in recent years. Negative terahertz photoconductivity reveals a plethora of fascinating ultrafast processes involving photoexcited states and unveil their unique intrinsic characteristics. This review highlights these unconventional responses of charge carriers and discusses the underlying physics for contemporary layered and one-dimensional materials. These understandings reveal extraordinary photophysical properties of materials which are essential for designing high-frequency advanced optoelectronic devices. - PublicationNear-infrared nanosecond-pulsed laser-activated highly efficient intracellular delivery mediated by nano-corrugated mushroom-shaped gold-coated polystyrene nanoparticles(14-06-2020)
; ;Kar, Srabani ;Chen, Te Chang ;Chen, Chih Wei ;Borana, Jayant ;Lee, Ming ChangTseng, Fan GangHere, an efficient intracellular delivery of molecules with high cell viability is reported using nanosecond-pulsed laser-activated plasmonic photoporation, mediated by high-aspect-ratio nano-corrugated mushroom-shaped gold-coated polystyrene nanoparticles (nm-AuPNPs) at near-infrared wavelength. Upon pulsed laser illumination, nm-AuPNPs exhibit greater plasmonic extinction than spherical AuPNPs, which increase their energy efficiency and reduce the necessary illumination of light, effectively controlling cell damage and improving the delivery efficiency. Nm-AuPNPs exhibit surface plasmon absorption at near infrared region with a peak at 945 nm. Pulsed laser illumination at this plasmon peak triggers explosive nanobubbles, which create transient membrane pores, allowing the delivery of dyes, quantum dots and plasmids into the different cell types. The results can be tuned by laser fluence, exposure time, molecular size and concentration of nm-AuPNPs. The best results are found for CL1-0 cells, which yielded a 94% intracellular PI dye uptake and ~100% cell viability at 35 mJ cm-2 laser fluence for 945 nm wavelength. Thus, the presented approach has proven to have an inevitable potential for biological cell research and therapeutic applications. - PublicationIntracellular Delivery using Anisotropic Gold Nanocrystals Synthesized by Microfluidic Device(27-09-2020)
;Illath, Kavitha ;Narasimhan, Ashwin Kumar ;Shinde, Pallavi ;Wankhar, Syrpailyne ;Nagai, MoetoThis work presents the synthesis of anisotropic gold nanocrystals (Au NCs) using a segmented flow-based microfluidic device, and intracellular delivery using the synthesized particles. The device consists of T and Y shape junctions, cross channel, and winding geometries, where reagents mix. Reagents meet at the initial Y-junction and the T-shape junction generates droplets. Synthesized Au NCs are characterized and are found to possess multiple plasmonic peaks as per their size, shape and anisotropic nature. These plasmonic peaks can be tuned to the near-infrared region, which is advantageous in different biomedical applications. With the synthesized Au NCs as a mediator, propidium iodide dye is successfully delivered into the cellular cytoplasm of HeLa cells by using nanosecond pulse laser. The results demonstrated that Au NCs synthesized using a segmented flow-based microfluidic device can be used for the intracellular delivery of different exogenous molecules. The best results are achieved as 96% delivery efficiency and 98% cell viability. Thus, our platform potentially applicable to anisotropic Au NCs synthesis as well as cellular therapy and diagnostic purpose. - PublicationSingle-Cell Manipulation(01-01-2021)
;Bhardwaj, Rohit ;Gupta, Harsh ;Pandey, Gaurav ;Ryu, Sangjin ;Shibata, Takayuki; Nagai, MoetoThe manipulation and analysis of single cells have enabled various applications in the field of medical and life sciences. Microfluidic single-cell manipulation uses designed flows in a microchannel network and plays an important role in precise and high-throughput manipulation of single cells. Unlike previous reviews and books covering a wide range of technologies, this chapter focuses on the basic mechanisms of hydrodynamic manipulation based on lumped modeling with circuit elements. First, a hydraulic circuit for cell manipulation is expressed as a fluidic resistance network by Hagen–Poiseuille’s law. Designing the ratio of flow rates between the cell trap and the bypass channels demonstrates single-cell trapping, which is extended to co-culture of single cells. Other forms of hydrodynamic manipulation, such as cell deforming, sorting, and separating, are highlighted. Several techniques combining hydrodynamic manipulation with other optical, electric, magnetic, and acoustic methods are developed to improve functionality. These cell manipulation examples are also explained in view of hydrodynamic modeling. Microchannels for hydrodynamic manipulation are designed independently of the other methods. By modeling the flow, the average flow can be predicted, and a microchannel network for hydrodynamic cell manipulation can be easily designed. - PublicationMassively parallel intracellular delivery using titanium oxide nanotubes(01-01-2020)
;Mohan, Loganathan ;Kar, Srabani ;Ren-Hattori, ;Ishii-Teshima, Miho ;Illath, Kavitha ;Tiwari, Anuj; ;Shibata, TakayukiNagai, MoetoThis paper reports titanium oxide nanotubes (TNTs)-based photoporation platform for versatile and highly efficient intracellular delivery, which is potentially applicable for cellular therapy and diagnostics. - PublicationRecent Advances of Biosensor-Integrated Organ-on-a-Chip Technologies for Diagnostics and Therapeutics(14-02-2023)
;Shinde, Ashwini ;Illath, Kavitha ;Kasiviswanathan, Uvanesh ;Nagabooshanam, Shalini ;Gupta, Pallavi ;Dey, Koyel ;Chakrabarty, Pulasta ;Nagai, Moeto ;Rao, Suresh ;Kar, Srabani - PublicationMicrofluidic platforms for single neuron analysis(01-01-2022)
;Gupta, Pallavi ;Shinde, Ashwini ;Illath, Kavitha ;Kar, Srabani ;Nagai, Moeto ;Tseng, Fan GangSingle-neuron actions are the basis of brain function, as clinical sequelae, neuronal dysfunction or failure for most of the central nervous system (CNS) diseases and injuries can be identified via tracing single-neurons. The bulk analysis methods tend to miscue critical information by assessing the population-averaged outcomes. However, its primary requisite in neuroscience to analyze single-neurons and to understand dynamic interplay of neurons and their environment. Microfluidic systems enable precise control over nano-to femto-liter volumes via adjusting device geometry, surface characteristics, and flow-dynamics, thus facilitating a well-defined micro-environment with spatio-temporal control for single-neuron analysis. The microfluidic platform not only offers a comprehensive landscape to study brain cell diversity at the level of transcriptome, genome, and/or epigenome of individual cells but also has a substantial role in deciphering complex dynamics of brain development and brain-related disorders. In this review, we highlight recent advances of microfluidic devices for single-neuron analysis, i.e., single-neuron trapping, single-neuron dynamics, single-neuron proteomics, single-neuron transcriptomics, drug delivery at the single-neuron level, single axon guidance, and single-neuron differentiation. Moreover, we also emphasize limitations and future challenges of single-neuron analysis by focusing on key performances of throughput and multiparametric activity analysis on microfluidic platforms. - PublicationSingle Biomolecule Detection and Analysis: Concepts, Applications, and Future Prospects(01-01-2023)
; Tseng, Fan GangThis collection discusses various micro/nanodevice design and fabrication for single-biomolecules detection. It will be an ideal reference text for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. This book-Discusses techniques of single-biomolecule detection, their advantages, limitations, and applications. Covers comprehensively several electrochemical detection techniques. Provides single-molecule separation, sensing, imaging, sequencing, and analysis in detail. Examines different types of cantilever-based biomolecule sensing, and its limitations. Single Biomolecule Detection and Analysis covers single-biomolecule detection and characterization using micro/nanotechnologies and micro/nanofluidic devices, electrical and magnetic detection technologies, microscopy and spectroscopy techniques, single biomolecule optical, and nanopore devices. This text covers key important biosensors-based detection, stochastic optical reconstruction microscopy-based detection, electrochemical detection, metabolic engineering of animal cells, single-molecule intracellular delivery and tracking, terahertz spectroscopy-based detection, total internal reflection fluorescence (TIFR) detection, and fluorescence correlation spectroscopy (FCS) detection. This text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. Discussing chemical process, physical process, separation, sensing, imaging, sequencing, and analysis of single-molecule detection, this text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. It covers microscopy and spectroscopy techniques for single-biomolecule detection, analysis, and biomedical engineering applications.