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Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium

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cris.author.scopus-author-id9036655500
cris.author.scopus-author-id7006104346
cris.virtual.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.author-orcid0000-0002-9671-5128
cris.virtual.department#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtual.departmentIndian Institute of Technology, Madras
cris.virtualsource.author-orcid#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.author-orcid927ffc6f-a3a3-44bf-b5f0-8e9b96314b5d
cris.virtualsource.department#PLACEHOLDER_PARENT_METADATA_VALUE#
cris.virtualsource.department927ffc6f-a3a3-44bf-b5f0-8e9b96314b5d
dc.contributor.authorAnjani Kumari, J.
dc.contributor.authorT Panda
dc.date.accessioned2023-09-20T06:42:02Z
dc.date.available2023-09-20T06:42:02Z
dc.date.issued01-01-1992
dc.description.abstractEfficient synthesis of a large number of viable protoplasts from Trichoderma longibrachiatum (Trichoderma reesei) mycelium has been found to be influenced by a number of factors. In this case, Trichoderma harzianum NCIM 1185 culture filtrate has been used to prepare protoplasts from Trichoderma longibrachiatum QM 9414. A method has been devised to isolate a large number of viable protoplasts from the mycelium. Detailed analysis of different factors affecting the synthesis of protoplasts from T. longibrachiatum has not yet been reported. The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml; mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzymes, 2 h; temperature of protoplasting, 30°C; phosphate buffer, 25 mm, pH 6.5; KCl as osmotic stabilizer, 0.7 m. Proper spreading of the mycelium in contact with the lytic enzymes was also found to be necessary. © 1992.
dc.identifier.doi10.1016/0141-0229(92)90073-W
dc.identifier.issn1410229
dc.identifier.scopus2-s2.0-0026832804
dc.identifier.urihttps://apicris.irins.org/handle/IITM2023/59999
dc.relation.ispartofseriesEnzyme and Microbial Technology
dc.sourceEnzyme and Microbial Technology
dc.subjectlytic enzymes
dc.subjectprotoplast
dc.subjectTrichoderma harzianum NCIM 1185
dc.subjectTrichoderma longibrachiatum QM 9414
dc.titleStudies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium
dc.typeJournal
dspace.entity.typePublication
oaire.citation.endPage248
oaire.citation.issue3
oaire.citation.startPage241
oaire.citation.volume14
oairecerif.author.affiliation#PLACEHOLDER_PARENT_METADATA_VALUE#
oairecerif.author.affiliationIndian Institute of Technology, Madras
person.affiliation.cityChennai
person.affiliation.id60025757
person.affiliation.nameIndian Institute of Technology Madras
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