Uniform Transfection: Shock Wave Generation in Laser Ablation and Microcontact Printing

With the increasing need of regenerative medicines, cell therapy, and biopharmaceutical drug, it is necessary to develop a method for massively parallel delivery and uniform transfection. This research explores the possibility of using \mu CP (using cell adhesive and cell blocking agents) for printing massive number of cells on a substrate and using optoporation methods (nano-second pulsed radiation) to perforate cell membranes. With combining these two methods, a stand-Alone micro-device capable of performing localized intracellular delivery will be developed. In this paper, we confirmed the generation of shock wave upon optoporation, tried intracellular delivery using Calcein, develop the stamp for microcontact printing and performed the printing.